Modern applications of proteomics techniques followed by bioinformatics analyses put the Center for Proteomics and Bioinformatics at the forefront of translational research in the Case Western Reserve University School of Medicine.
New Instruments Coming Soon!
In Summer 2012, two new Waters NanoAcquity UPLCs interfaced to Thermo Orbitrap Elites equipped with collisionally activated dissociation (CAD) sources for tandem mass spectrometry experiments will be installed and operational. The Waters NanoAcquity UPLC affords up to four hour gradients. These gradients are useful in resolving complex mixtures commonly found in clinical proteomic samples.
The Thermo Orbitrap Elite mass spectrometers will scan up to four times faster than their Orbitrap Velos counterpart, increasing the number of proteins identified. The higher resolution of these instruments allows for more accurate quantitation. Long gradient length and fast scan speed features of these instruments make them ideal for clinical proteomic studies.
If you would like to utilize our new instruments for your research needs, please contact Janna Kiselar at 368-0979 or janna.kiselar@case.edu.
Structural Biology and Biophysics Training Program (SBB_TP)
The Structural Biology and Biophysics Training Program emphasizes quantitative methods and equips students to study cell and protein structure and function using state-of-the-art instrumentation and computing. The Department hosts outstanding research programs in the areas of structural biology and cellular ion channels and transporters.
Qualified students receive full tuition waiver, a stipend, and health benefits during the full duration of their studies.
Students can enter this training program by admission to the Interdepartmental Structural Biology and Biophysics Training Program (http://sbb-tp.case.edu).
Endogenous protein S-nitrosylation in E. coli: regulation by OxyR
Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in E. coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, >>
Insights into substrate specificity and metal activation of mammalian tetrahedral aspartyl aminopeptidase
Aminopeptidases are key enzymes involved in the regulation of signaling peptide activity. Here we present a detailed biochemical and structural analysis of an evolutionary highly conserved aspartyl aminopeptidase called DNPEP. >>
Joint adaptive mean-variance regularization and variance stabilization of high dimensional data
The paper addresses a common problem in the analysis of high-dimensional high-throughput "omics" data, which is parameter estimation across multiple variables in a set of data where the number of variables is much larger than the sample size. >>