Instruments and Computational Facilities

Computational Facilities
Mass Spectrometry Equipment
Biochemistry Facilities

Computational Facilities

  1. Genologics Proteus LIMS for automated data acquisition, analysis.
  2. Clinical Barcoding System 
  3. Data acquisition computers attached to mass spectrometers, scanners, Beckman BioMex FX robot, HPLCs and other analytical equipments.
  4. Data analysis software:
    1. Rosetta Biosoftware Elucidator
    2. Matrix Science Mascot
    3. Tibco Spotfire
    4. GE Healthcare DeCyder
    5. Ingenuity Pathway Analysis (IPA)
    6. Proteome Software Scaffold
    7. X! Tandem
    8. Sequest
    9. ProtMapMS
    10. MassMatrix
  5. Dedicated, remote access data analysis workstations including 2 workstations with BioWorks for mass spectrometry (MS) database searches, 2 workstations with DeCyder 2D software for 2D-DIGE data analyses.
  6. Closed-circuit camera system for trouble shooting and user instructions of Mass Spectrometers with computer recording capability.

Mass Spectrometry Equipment

The Proteomics Core laboratory has a full complement of mass spectrometry and proteomics facilities available including:

  1. Waters nanoACQUITY UPLC interfaced to LTQ Orbitrap Velos.
  2. Dionex Ultimate 3000 RSLC nano LC system interfaced to a Thermo TSQ Quantum Ultra
  3. GE/Amersham 2-D gel DIGE system with robotic spot picking.
  4. Beckman Biomec FX robotic liquid handling systems is available with 96-well head and SPAN-8 flexible arm. Numerous ALPs are available including heating/cooling and vacuum filtration.  
  5. 2 Waters nanoACQUITY UPLC interfaced to Orbitrap Elites

Other major equipment includes: Protein solutions light scattering instrument, time-resolved fluorescence using CCD detection, FTIR, and Uv-Vis spectrometers.

Biochemistry Facilities

The Center hosts a variety of biochemistry facilities and equipment including: HPLC and FPLC chromatography apparatuses, microfuges, regulated water baths, gel dryer, vacuum pump, high voltage power supplies, sequencing gel units, protein and DNA electrophoresis apparatuses, refrigerators, freezers, analytical balances, pH meter, micropipets, radiation safety equipment, glassware, pipettes, -80 degree Celsius freezers and cold rooms.