Center for Proteomics and Bioinformatics

Instruments and Computational Facilities

Instrument Schedule
Computational Facilities
Mass Spectrometry Equipment
Biochemistry Facilities

Instrument Schedule

Red = Orbitrap
Purple = Orbitrap Velos
Blue = MALDI
Orange = FT-LTQ
Green = TSQ Triple Quad

Computational Facilities

  1. Genologics Proteus LIMS for automated data acquisition, analysis.
  2. Clinical Barcoding System 
  3. Data acquisition computers attached to mass spectrometers, scanners, Beckman BioMex FX robot, HPLCs and other analytical equipments.
  4. Data analysis software:
    1. Rosetta Biosoftware Elucidator
    2. Matrix Science Mascot
    3. Tibco Spotfire
    4. GE Healthcare DeCyder
    5. Ingenuity Pathway Analysis (IPA)
    6. Proteome Software Scaffold
    7. X! Tandem
    8. Sequest
    9. ProtMapMS
  5. Dedicated, remote access data analysis workstations including 2 workstations with BioWorks for mass spectrometry (MS) database searches, 2 workstations with DeCyder 2D software for 2D-DIGE data analyses.
  6. Closed-circuit camera system for trouble shooting and user instructions of Mass Spectrometers with computer recording capability.

Mass Spectrometry Equipment

The Proteomics Core laboratory has a full complement of mass spectrometry and proteomics facilities available including:

  1. Thermo-Finnigan Fourier Transform LTQ mass spectrometer equipped with 7 T superconductive magnet with ppm resolution for top-down and bottom-up proteomics capabilities operated with Dionex capillary LC system; a 96-well autosampler and switcher is utilized with this system.
  2. Thermo-Finnigan LTQ-OT-11000 LTQ Orbitrap ETD with a Dionex Utimate 3000 parallel HPLC system.
  3. LTQ Orbitrap Velos with nanoACQUITY UPLC (Waters).
  4. GE/Amersham 2-D gel DIGE system with robotic spot picking.
  5. Beckman Biomec FX robotic liquid handling systems is available with 96-well head and SPAN-8 flexible arm. Numerous ALPs are available including heating/cooling and vacuum filtration.  
  6. Pro-TOF 2000 MALDI Mass Spectrometer, with attamole sensitivity and 5 ppm resolution.

Other major equipment includes: Protein solutions light scattering instrument, time-resolved fluorescence using CCD detection, FTIR, and Uv-Vis spectrometers.

Biochemistry Facilities

The Center hosts a variety of biochemistry facilities and equipment including: HPLC and FPLC chromatography apparatuses, microfuges, regulated water baths, gel dryer, vacuum pump, high voltage power supplies, sequencing gel units, protein and DNA electrophoresis apparatuses, refrigerators, freezers, analytical balances, pH meter, micropipets, radiation safety equipment, glassware, pipettes, -80 degree Celsius freezers and cold rooms.