Frequently Asked Questions (FAQ)

I have a set of candidate genes/proteins, and I'm interested in doing a systems-level pathway analysis of the data. Can you help me with that?
How much does it cost to use the services in your Center?
How can I receive more information about the Center's seminars, workshops and other events?
What is the best way to submit a sample to your Center?
Is the CCPB available for use by researchers outside of the Center and/or Case Western Reserve University?
What type of facilities do you have at your Center for Synchrotron Biosciences at Brookhaven National Laboratory at the National Synchrotron Light Source in New York?
How many researchers have used your Centers they started operations?


Q: I have a set of candidate genes/proteins, and I'm interested in doing a systems-level pathway analysis of the data. Can you help me with that?

A: Certainly! The Center provides pathway analysis services using both commerical software (MetaCore and Ingenuity Pathway Analysis), as well as technology developed in house. We are also developing methods to couple various high-throughput datasets (e.g. 2D DIGE + microarray data; SNP data + microarray data) to guide hypothesis development towards the most biologically functional parts of the system.

Q: How much does it cost to use the services in your Center?

A: See our price sheet.

Q. I have a 1D gel band that I would like to identify the proteins from. What is the cost to do this?

A: For this type of sample we would perform a digest, analyze with a short gradient and then run a database search. You would then receive a report indicating the proteins identified within a given cut off.

Digestion$10
LC/MS/MS Short Gradient$180
Data Analysis (3 hrs included)$0
Total$190 per sample

Q: We are interested in changes that may occur in the liver of mice +/- drug treatment. How much would this cost?

A: Please contact Daniela Schlatzer to discuss feasibility at 216-368-4014 or daniela.schlatzer@case.edu.

Q: We are interested in Phosphoproteome changes in cells that have been exposed to hypoxia. How much would this cost?

A: Please contact Janna Kiselar at 216-368-0979 or janna.kiselar@case.edu to discuss the project and pricing.

Q: How can I receive more information about the Center's seminars, workshops and other events?

A: You can request to join our email distribution list by sending an email to maita.diaz@case.edu.

Q: What is the best way to submit a sample to your Center?

A: Investigators may "drop-off" samples for service or they may bring their samples to the CPB and run them independently on the CPB.s instruments. Independent investigators are trained by Center staff in instrument operation, data retrieval and data analysis. If you would like to perform 2D Gel work, please contact Elizabeth Yohannes. If you would like to perform Mass Spectrometry work or be trained on our instruments, please contact Janna Kiselar.

Q: Is the CCPB available for use by researchers outside of the Center and/or Case Western Reserve University?

A: The Case Center for Proteomics and Bioinformatics (CPB) facility is available to all investigators at Case Western Reserve University, University Hospitals, MetroHealth System, the Cleveland Clinic Research Institute, and the Cleveland Clinic Foundation. Depending on research needs and availability, the Center.s research core facilities may also be available to researchers at other non-profit or for-profit research institutions across the country.

Many investigators and projects require a close collaboration with CCPB faculty and staff members. CCPB members work closely with collaborators to design and run experiments as well as analyze data, publish results and submit grant proposals.

Q: What type of facilities do you have at your Center for Synchrotron Biosciences at Brookhaven National Laboratory at the National Synchrotron Light Source in New York?

A: Three of our research cores (Macromolecular Crystallography, X-ray Spectroscopy, and X-ray Footprinting) are available to a national community of users at the Center for Synchrotron Biosciences (CSB) which operates 4 beamlines. Please visit the CSB Website to learn more about the X29A, X3A, X3B and X28C beamlines.

Q: How many researchers have used your Centers since they started operations?

A: Overall, 37 Departments and Centers from Case Western Reserve University and 86 institutions worldwide have utilized the Case Center for Proteomics and Bioinformatics and the Case Center for Synchrotron Biosciences.