New pricing effective Feb. 1, 2012!
| Preparatory Services | |
| 1D Gel Electrophoresis Up to 9 samples per gel | $65 |
| Protein Concentration Analysis (per sample) | $35 |
| Sample Clean-up (per sample) | $35 |
| Affinity Separation (per sample) Agilent Hu-14 | $75 |
| C18 Processing (per sample) | $15 |
| Digestion (per sample) Includes reduction, alkylation and enzyme | $10 |
| Post-translational Modification and Structural Analysis | |
| Global Phosphoproteome Analysis Includes digestion, enrichment, LC/MS/MS and analysis | Call for Quote |
| Phosphorylation ID (per sample) Includes digestion, enrichment, LC/MS/MS and analysis | $500 |
| Footprinting Analysis (per sample) Includes digestion, LC-MS/MS and analysis | $130 |
| LC/MS/MS Analysis | |
| Short Gradient (per sample) | $290 |
| Long Gradient (per sample) | $365 |
| ELISA Analysis | |
| ELISA (per plate) Includes plate, analysis and report generation | $1500 |
| Quantitative Analysis | Call for Quote |
| Label Free | |
| iTRAQ | |
| SILAC | |
| Heavy Peptide | |
| Multiple Reaction Monitoring (MRM) | |
| Intact Mass Analysis | |
| FT-LTQ or MALDI-TOF (per sample) | $65 |
| Independent Instrument Usage | |
| MALDI-TOF | $45 per 1/2 hr or $65 per hr |
| Off-Line HPLC (per sample) | $105 |
| Off-Line FPLC (per sample) | $50 |
| Data Analysis Services | |
| Molecular and Systems Biology and Standard Data Analysis | $80 per hr |
| Instrument Training | |
| MALDI-TOF Contact Center to Schedule | $250 |
| 2D Gel Services | |
| Deep Purple Staining (per sample) Includes scanning | $250 |
| Gel Scanning using Typhoon Imager | $60 per gel |
| Independent Typhoon Usage | $52 per hr |
| Spot Picking for Digestion | $105 |
| 2D Gel Run Without Labeling | $110 |
| Cell Pellet Tissue Processing (per sample) 2D Gel Sample Processing | $220 |
| Run and Image 1 Sample in One Gel Includes labeling | $350 |
| Run and Image 2 Samples in One Gel Includes labeling | $460 |
| Run and Image 3 Samples in One Gel Includes labeling | $570 |
| 95 Well MALDI Service Includes raw data and images | $1575 |
Investigators are highly encouraged to contact Janna Kiselar janna.kiselar@case.edu for CTSC grants and Daniela Schlatzer Daniela.schlatzer@case.edu for quantitative projects. We are happy to discuss experimental design and provide any support/ assistance for your project.
Q. I have a 1D gel band that I would like to identify the proteins from. What is the cost to do this?
A: For this type of sample we would perform a digest, analyze with a short gradient and then run a database search. You would then receive a report indicating the proteins identified within a given cut off.
| Digestion | $10 |
| LC/MS/MS Short Gradient | $290 |
| Data Analysis (3 hrs included) | $0 |
| Total | $300 per sample |
Q: We are interested in changes that may occur in the liver of mice +/- drug treatment. How much would this cost?
A: Please contact Daniela Schlatzer to discuss feasibility at 216-368-4014 or daniela.schlatzer@case.edu.
Q: We are interested in Phosphoproteome changes in cells that have been exposed to hypoxia. How much would this cost?
A: Please contact Janna Kiselar at 216-368-0979 or janna.kiselar@case.edu to discuss the project and pricing.
If you would like to submit a sample to our Center, please download the Project Submission form and follow the instructions on the front page.