2D-Dige

Provides accurate quantitative information about response to stimulation through separation of proteins by charge and size

Advantages

  • Accurate quantitation
  • The only top down platform
  • High resolving power of samples at protein level
  • Each sample within gel can be normalized
  • Abundance of each protein spot can be separated from the biological variation
  • Incorporates internal standard (every protein represented)

Disadvantages

  • This technique requires large amounts of protein
  • Difficulty identifying membrane proteins

Contact Person

Elizabeth Yohannes, Ph.D. (elizabeth.yohannes@case.edu)
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue BRB 935, Cleveland, Ohio 44106
phone: 216-368-0655 fax: 216-368-6846

2D-DIGE Review

Marouga R., et al.. The development of the DIGE system: 2D fluorescence difference gel analysis technology. Anal Bioanal Chem 2005;382:669-678, doi: 10.1007/s00216-005-3126-3.

Center Paper

Yohannes E, et al. Proteomic Signatures of Human Oral Epithelial Cells in HIV-Infected Subjects. PLoS ON 2011;E 6(11): e27816. doi:10.1371/journal.pone.0027816

Relative Quantification

Absolute Quantification