Post-Translational Modification (PTM) proteomics is the study of covalent chemical modification of a protein after its translation; PTMs can be a function of stimulation or condition (disease, time, drug, etc.). Mass spectrometry is superior to other techniques for identifying PTMs. The main advantage of this technique is its ability to not only identify the PTM with sub ppm mass accuracy but also to localize the site(s) of the modification. The Center has experience analyzing many PTMs including Phosphorylation, Acetylation, Nitrosylation, Glycosylation and Methylation. For your convenience, the pages that follow provide detailed workflows and information regarding each technique.