Acetylpeptide Mass Spectrometry Analysis

Advantages

  • MS enables you to map the exact site of modification
  • High-throughput analysis
  • High-sensitivity

Disadvantages

  • Requires more protein than immunoblot detection
  • Preferential enrichment of peptides resulting from high abundant proteins.

Contact Person

Janna Kiselar, Ph.D. (janna.kiselar@case.edu)
Instructor
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-0979
fax: 216-368-6846

Acetylproteomic MS:

Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14; 325(5942):834-40

Dormeyer, W., et al. Probing Lysine Acetylation in Proteins Strategies, Limitations, and Pitfalls of in Vitro Acetyltransferase Assays, Mol. Cell Prot. 2005. 4: 1226-1239. doi: 10.1074/mcp.M500047-MCP200

Center Paper

Kadiyala, C., et al. Acetylation of Retinal Histones in Diabetes Increases Inflammatory Proteins., J. Bio. Chem. 2012. 278: 25869-25880. doi: 10.1074/jbc.M112.375204

Post Translational Modifications Proteomics