ELISA

Uses monoclonal antibodies to accurately detect and measure specific proteins in biological samples.

Advantages

  • Uses monoclonal antibodies to accurately detect and measure specific proteins in biological samples.

Disadvantages

  • Must have a known target protein
  • Some detergents may interfere
  • Not cost effective for very small sample sizes
  • Commercial kits not available for all antigens

Contact Person

Danie Schlatzer (daniela.schlatzer@case.edu)
Senior Research Associate
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-4014 fax: 216-368-6846

ELISA Review

Premjeet, Sandhu, et al. Enzyme-Linked Immuno-Sorbent Assay (ELISA), basics and it's application: A comprehensive review. Journal of Pharmacy Research. 2011, 4 (12) 4581-4583.

Center Paper

Schlatzer DM, et al. Human biomarker discovery and predictive models for disease progression for idiopathic pneumonia syndrome following allogeneic stem cell transplantation. Mol Cell Proteomics. 2012;11(6): doi: 10.1074/mcp.M111.015479.

Relative Quantification

Absolute Quantification