Methylpeptide Mass Spectrometry Analysis

Advantages

  • MS enables you to map the exact site of modification
  • High-throughput analysis
  • High-sensitivity

Disadvantages

  • Requires more protein than immunoblot detection
  • Preferential enrichment of peptides resulting from high abundant proteins

Contact Person

Janna Kiselar, Ph.D. (janna.kiselar@case.edu)
Instructor
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-0979
fax: 216-368-6846

Methylproteomic MS:

Erce, M., et al. The methylproteome and the intracellular methylation network., Proteomics. 2012. 12: 564-586. doi: 10.1002/pmic.201100397

Center Paper

Yang, J., et al. Reversible methylation of protomter-bound STAT3 by histone-modifying enzymes., PNAS. 2010. 14: 21499-504. doi: 10.1073/pnas.1016147107/-/DCSupplemental

Post Translational Modifications Proteomics