MRM

Advantages

  • Reliable absolute quantification of proteins/peptides of low abundance in complex sample matrix
  • Multiplexed assay platform make it an ideal tool for biomarker verification
  • High sensitivity, selectivity, and dynamic range make this technique well suited for the targeted detection
  • High precision and reproducibility

Disadvantages

  • Synthesized peptides are costly if there are many peptides need to be quantified.

Contact Person

Danie Schlatzer (daniela.schlatzer@case.edu)
Senior Research Associate
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-4014 fax: 216-368-6846

MRM Review

Vinzenz Lange, et al. Selected reaction monitoring for quantitative proteomics: a tutorial., Molecular Systems Biology. 2008 1-14. Article number 222; doi: 10.1038/msb.2008.61

Relative Quantification

Absolute Quantitation