Nitrosylpeptide Mass Spectrometry Analysis

Advantages

  • MS enables you to map the exact site of modification
  • High-throughput analysis
  • Enrichment at protein level
  • High-sensitivity

Disadvantages

  • Requires more protein than immunoblot detection

Contact Person

Janna Kiselar, Ph.D. (janna.kiselar@case.edu)
Instructor
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-0979
fax: 216-368-6846

Nitrosylproteomic MS:

Forrester, M., et al. Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture., Nature Biotechnology. 2009. 27: 557-559. doi: 10.1038/nbt.1545

Center Paper

Seth, D., et al. Endogenous protein S-Nitrosylation in E. coli: regulation by OxyR., Science. 2012. 336: 470-3. doi: 10.1126/science.1215643

Post Translational Modifications Proteomics