Phosphopeptide Mass Spectrometry Analysis

Advantages

  • MS enables you to map the exact site of modification
  • Allows for quantitative determination of phosphorylation status in cell
  • High-throughput analysis
  • High-sensitivity

Disadvantages

  • Requires more protein than immunoblot detection
  • Preferential enrichment of peptides resulting from high abundant proteins.

Contact Person

Janna Kiselar, Ph.D. (janna.kiselar@case.edu)
Instructor
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-0979
fax: 216-368-6846

Phosphoproteomic MS:

Macek, B., et al. Global and Site-Specific Quantitative Phosphoproteomics: Principles and Applications, Annu. Rev. Pharmacol. Toxicol. 2009. 49: 199-221. doi: 10.1146/annurev.pharmtox.011008.145606

Center Paper

Bulek, K., et al. The Inducible kinase IKKi is required for IL-17-dependent signaling associated with neutorphilia and pulmonary inflammation., Nature Immunology. 2011. 12: 844-852. doi: 10.1038/ni.2080

Post Translational Modifications Proteomics