Western Blot

Uses antibodies to accurately detect and measure specific proteins in biological samples.

Advantages

  • Highly specific for a particular protein
  • Detects antigen as a protein, rather than peptides
  • Can confirm presence and quantity of a specific protein
  • Minimal sample processing necessary in most cases

Disadvantages

  • Must have a known target protein
  • Some detergents may interfere
  • Not cost effective for very small sample sizes

Contact Person

Danie Schlatzer (daniela.schlatzer@case.edu)
Senior Research Associate
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-4014 fax: 216-368-6846

Relative Quantification

Absolute Quantification