X-Ray Footprinting MS

Advantages

  • Can accommodate membrane proteins
  • Good for macromolecular assemblies that cannot be crystallized or are too big for NMR
  • Provides information about interactions of bulk proteins, bound proteins and how water orders itself
  • Provide protein and water dynamics information

Disadvantages

  • Need purified protein

Contact Person

Janna Kiselar, Ph.D. (janna.kiselar@case.edu)
Instructor
Case Center for Proteomics and Bioinformatics
Case Western Reserve University
10900 Euclid Avenue, BRB 947 Cleveland, Ohio 44106
phone: 216-368-0979
fax: 216-368-6846

X-Ray Footprinting Review:

Takamoto, K., et al.. Radiolytic Protein Footprinting with Mass Spectrometry to Probe the Structure of Macromolecular Complexes Annual Rev BioChem 2006;35:251-76. doi: 10.1146/annurev.biophys.35.040405.102050

Center Paper

Kiselar, J., et al. : Structural Analysis of Proinsulin Hexamer Assembly by Hydroxyl Radical Footprinting and Computational Modeling J. Bio. Chem. 2011; 286(51): 43710-16. doi:10.1074/jbc.M111.297853

Structural Proteomics